Functional Diagnostic Testing

RH+ offers the best and the most advance diagnostic testing in India from three different labs in USA. These labs specialize in various functional diagnostic testing as well genomic testing to help clinicians and health practitioners diagnose, cure and prevent chronic diseases. Diagnostic labs in India are not equipped in these kinds of testing but these labs offer tests that combine standard and innovative biomarkers designed to provide a complete understanding of specific biological systems. Genomic testing helps you modulate your lifestyle and nutritional insufficiencies which would otherwise could be detrimental to health in future. Genomic testing helps to test various genetic mutations, variations or polymorphism responsible for various types of cancers, heart disease, liver disease or autoimmune disorders.

Genova Diagnostics:                                                Print

Genova diagnostic is located in Asheville, NC USA and have their presence throughout the world. Genova specialises in clinical chemistry, bacteriology, mycology, parasitology, toxicology as well as molecular genetics. We offer following tests from Genova testing:
. Allergix IgG4 Food Antibodies Profile Serum/Blood spot
. Comprehensive Thyroid Assessment
. Intestinal Permeability Assessment
. Organix Urine Comprehensive Profile Adult/Child
. Fatty acids Profile-Blood spot

Doctors Data:                                  DD_logo_Sponsor

Doctors Data also have global presence with headquarters in Illinois, USA. DD specializes in heavy metal testing, gut microbiology and cardiovascular profile. We offer the following tests from DD labs:
. Comprehensive Stool Analysis with parasitology
. RBC elements profile
. Hair toxic elements exposure profile
. Celiac and Gluten Sensitivity

ZRT lab:                            ZRT

ZRT lab specialises in salivary hormones and dried urine testing for free hormones and urinary metabolites. We offer following tests from ZRT labs:
. Adrenal Stress profile
. Male/Female Hormonal Profile

Genova Diagnostics

1) Allergix IgG4 Food Antibodies Profile Serum/Blood spot

The Allergix® IgG4 Food Antibodies 90 is a food sensitivity test, which helps in identifying those with IgG-mediated food intolerances. IgG4 antibody is related to delayed or non-atopic food reactions that exacerbate or contribute to many different health problems. This antibody food sensitivity test is ideal for patients who suffer from delayed reactions/sensitivities to specific foods.

What is an IgG4 food sensitivity response?

An IgG4 response to food is actually more common than the IgE response, which causes an immediate reaction. These food sensitivity reactions are more difficult to notice since they can occur hours or even days after consumption of an offending food. In some cases, a person’s reaction to a food may occur several days after eating the offending food and the link between the food and their symptoms may not be connected. These hidden food “allergies” are actually food “sensitivities” and are caused by increasing blood levels of IgG4 antibodies in reaction to specific foods. This profile measures the IgG4 levels in serum that react to 90 different foods, including commonly eaten foods such as corn, milk, egg, and wheat. A food intolerance reaction patient guide is provided with the result of each test.

2) Comprehensive Thyroid Assessment

The Comprehensive Thyroid Assessment is a hormone test that provides a thorough analysis of thyroid hormone metabolism. It includes central thyroid gland regulation and activity, thyroid production and secretion, peripheral thyroid conversion, and thyroid autoimmunity. This hormone test allows the practitioner to pinpoint common imbalances that underlie a broad spectrum of chronic illness. This test analyses serum levels of TSH, free T4, free T3, reverse T3, anti-TG antibodies, and anti-TPO antibodies to assess central and peripheral thyroid function, as well as thyroid auto-immunity.
Thyroid hormones are essential and primary regulators of the body´s metabolism. Hormone imbalances can affect virtually every metabolic process in the body, exerting significant effects on mood and energy level. The Comprehensive Thyroid Assessment is a hormonal test which reveals imbalances that often go undetected with more limited assessments.

This thyroid hormone testing measures:
• Unbound levels of T4 and T3 which reflect the bioactive portion of thyroid hormone. This hormone assessment can identify not only overt hyper-and hypothyroidism, but subtle sub-clinical manifestations of thyroid dysfunction.
• Reverse T3, levels of which can increase when peripheral conversion to T4 to active T3 is impaired. Peripheral thyroid imbalances may arise from nutrient shortages, heavy metal exposure, adrenal stress, enzyme deficiencies, and other chronic illness.
• Thyroid antibody levels, which help gauge autoimmune response and may reflect metabolic irregularities and hypothyroidism even when TSH and T4 levels appear normal. Thyroid antibody levels may rise in response to trauma, dysbiosis, inflammation (including thyroiditis) or progressive thyroid degeneration.

3) Intestinal Permeability Assessment

Intestinal Permeability Assessment is a powerful noninvasive gastrointestinal test assessment of small intestinal absorption and barrier function in the bowel. The small intestine uniquely functions as a digestive/absorptive organ for nutrients as well as a powerful immune and mechanical barrier against excessive absorption of bacteria, food antigens, and other macromolecules. Both malabsorption and increased intestinal permeability (“leaky gut”) are associated with chronic gastrointestinal imbalances as well as many systemic disorders. Increased intestinal permeability (leaky gut) of the small intestine can:
• Increase the number of foreign compounds entering the bloodstream
• Allow bacterial antigens capable of cross-reacting with the host tissue to enter the bloodstream, leading to auto-immune processes
• Enhance the uptake of toxic compounds that can overwhelm the hepatic detoxification system and lead to an overly sensitive immune system

Leaky gut has been observed in a range of disorders such as:
• Inflammatory Bowel Disease (IBD)
• Food allergy
• Inflammatory joint disease
• Chronic dermatologic conditions

4) Organix Urine Comprehensive Profile Adult/Child

The Organix® Comprehensive Profile is a nutritional test providing insights into organic acids and a view into the body’s cellular metabolic processes. Additionally, children’s reference ranges are designed to provide more accurate pediatric nutritional evaluations.

Organic acids are metabolic intermediates that are produced in pathways of central energy production, detoxification, neurotransmitter breakdown, or intestinal microbial activity. Marked accumulation of specific organic acids detected in urine often signals a metabolic inhibition or block. The metabolic block may be due to a nutrient deficiency, an inherited enzyme deficit, toxic build-up or drug effect. Several of the biomarkers are markers of intestinal bacterial or yeast overgrowth.

The Organix® Comprehensive nutritional test profile provides vital patient information from a single urine specimen. This organic acids nutritional test is valuable for determining:

• Functional vitamin and mineral status
• Amino acid insufficiencies like carnitine and NAC
• Oxidative damage and antioxidant need
• Phase I & Phase II detoxification capacity
• Functional B-complex vitamin need
• Neurotransmitter metabolites
• Mitochondrial energy production
• Methylation sufficiency
• Lipoic acid and CoQ10 status
• Markers for bacterial and yeast overgrowth

5) Fatty acids profile – blood spot

From a simple finger stick it measures key omega-3 and omega-6 fatty acids and calculates key indicators to establish your optimal balance. Trans fatty acids the “bad” oils in processed foods are also measured.
• Improve patient compliance – Testing guides and supporting your recommendations for higher doses of fatty acid supplements.
• Inflammatory balance – Improper fatty acid intake affects the balance of anti- and pro-inflammatory eicosanoids, increasing health risks.
• Statins – Among the top five drugs prescribed last year, statins have shown to unfavourably alter this inflammatory balance.
• Increased free radical production – Consumption of polyunsaturated fatty acids (PUFAs) without increasing antioxidant intake will cause increased production of free radicals.

Fatty Acids measured:

Polyunsaturated Omega-3
• ALA (Alpha-Linolenic Acid)
• EPA (Eicosapentaenoic Acid)
• DHA (Docosahexaenoic Acid)

Polyunsaturated Omega-6
• LA (Linoleic Acid)
• GLA (Gamma Linolenic Acid)
• DGLA (Dihomogamma Linolenic Acid)
• AA (Arachidonic Acid)

Trans
• Total C:18 Trans

Indicators calculated:
• AA : EPA ratio – A measure of “silent” inflammation
• EPA + DHA % – An early CVD risk indicator
• LA : GLA Ratio – Delta-6 desaturase inhibition by decreased Zinc or decreased Magnesium, increased insulin, or dietary excess of saturated or trans fatty acids
• EPA : DGLA Ratio – Helps balance intake of Series-3 and Series-1 eicosanoid precursors

Doctor’s Data

1) Comprehensive Stool Analysis with parasitology

The Comprehensive Stool Analysis with Parasitology x1, 2, or 3 is an invaluable non-invasive diagnostic assessment that permits practitioners to objectively evaluate the status of beneficial and imbalanced commensal bacteria, pathogenic bacteria, yeast/fungus and parasites. Precise identification of pathogenic species and susceptibility testing greatly facilitates selection of the most appropriate pharmaceutical or natural treatment agents. Important information regarding the efficiency of digestion and absorption can be obtained from the measurement of the fecal levels of elastase (pancreatic exocrine sufficiency), fat, muscle and vegetable fibers, and carbohydrates. Inflammation can significantly increase intestinal permeability and compromise assimilation of nutrients. The extent of inflammation, whether caused by pathogens or inflammatory bowel disease (IBD), can be assessed and monitored by examination of the levels of biomarkers such as lysozyme, lactoferrin, white blood cells and mucus. These markers can be used to differentiate between inflammation associated with potentially life-threatening inflammatory bowel disease (IBD), which requires lifelong treatment, and less severe inflammation that can be associated with irritable bowel syndrome (IBS) which is frequently due to the presence of enteroinvasive pathogens. Lactoferrin is the only markedly elevated prior to and during the active phases of IBD, but not with IBS. Monitoring fecal lactoferrin levels in patients with IBD can therefore facilitate timely treatment of IBD, and the test can be ordered separately. Since the vast majority of secretory IgA (sIgA) is normally present in the GI tract, where it prevents binding of pathogens and antigens to the mucosal membrane, it is essential to know the status of sIgA in the gut. sIgA is the only bona fide marker of humoral immune status in the GI tract. Cornerstones of good health include proper digestion of food, assimilation of nutrients, and exclusion of pathogens and timely elimination of waste. To obtain benefits from food that is consumed, nutrients must be appropriately digested and then efficiently absorbed into portal circulation. Microbes, larger-sized particles of fiber, and undigested foodstuffs should remain within the intestinal lumen. Poor digestion and mal-absorption of vital nutrients can contribute to degenerative diseases, compromised immune status and nutritional deficiencies. Impairment of the highly specific nutrient uptake processes, or compromised GI barrier function, as in “leaky gut syndrome,” can result from a number of causes including:

. Low gastric acid production

. Chronic maldigestion

. Food allergen impact on bowel absorptive surfaces

. Bacterial overgrowth or imbalances (dysbiosis)

. Pathogenic bacteria, yeast or parasites and related toxic irritants

Click here for Sample CDSA report

2) RBC Elements Profile

Red blood cell (RBC) analysis is an invaluable method for assessing insufficiency or excess of elements that have important functions within cells or on blood cell membranes. An important feature of this analysis is that the cells are not washed, because this would result in partial loss of some important elements, such as calcium, that bind to the plasma membrane.

RBC element levels are very useful for:
– Cardiotonic influences (magnesium, potassium)
– Anti-inflammatory processes (selenium, copper, zinc)
– Anemia (copper, iron)
– Immunological function (zinc, copper, magnesium)
– Glucose tolerance (chromium, manganese and possibly vanadium)

Disorders specifically associated with zinc deficiency are also be addressed by this analysis. These disorders include loss of visual acuity, dysgeusia, dermatitis and poor wound healing, alopecia, amino acid mal-absorption, sexual impotence, decreased production of testosterone, depressed immune function and growth retardation.

Accurate assessment of essential element status is highly recommended for the determination of appropriate supplementation. The absorption, transport and metabolism of essential elements is highly integrated and regulated. Inappropriate supplementation or dietary imbalance of elements can have significant adverse health effects. For example, excess intake of zinc or molybdenum can result in copper deficiency and, although essential, excess retention of manganese can have serious neuro-toxic effects.

RBC element analysis is also useful for the assessment of ongoing or recent exposure to specific toxic elements that accumulate preferentially in the erythrocytes. These toxic elements include Arsenic, Cadmium, Lead, Methylmercury and Thallium. It is important to keep in mind that elevated levels of the toxic elements in these cells reflect only recent or ongoing exposure and do not provide information about the net retention of the metals in the body.

3) Hair Toxic Elements Profile

Toxic elements may be 200 to 300 times more highly concentrated in hair than in blood or urine. Therefore, hair is the tissue of choice for detection of recent exposure to elements such as arsenic, aluminium, cadmium, lead, antimony and mercury. The CDC acknowledges the value of hair mercury levels as a maternal and infant marker for exposure to neurotoxic methylmercury from fish. Hair Elements analysis provides information regarding recent and ongoing exposure to potentially toxic metals, especially methylmercury and arsenic, and time-averaged status of specific nutrient elements.

4) Celiac and Gluten Sensitivity

Celiac disease (CD) is often undiagnosed and is caused in genetically predisposed individuals by abnormal intestinal permeability and abnormal immune response to gluten, a protein complex found in wheat, barley, spelt and rye. The inflammatory autoimmune response damages the lining of the small bowel and is associated with diarrhoea, bloating, fatigue, nutritional deficiencies, and systemic autoimmune conditions. Gluten sensitivity can cause similar symptoms but without the same level of tissue damage. The Celiac & Gluten Sensitivity profile from Doctor’s Data helps in differentiating between CD, gluten sensitivity and wheat allergy by evaluating the serum titres of IgA and IgG for tissue transglutaminase, deaminated gliadin peptide, and gliadin. Wheat allergy is assessed by titres of IgE for wheat.

Celiac Disease
CD is also associated with other clinical disorders including thyroiditis, type I diabetes mellitus, Down syndrome, and IgA deficiency.  Patients diagnosed with CD must remain on a gluten-free diet for life and avoid all gluten containing foods and grains (wheat, rye, spelt, barley).

Non-Celiac Gluten Sensitivity (NCGS)
Individuals with NCGS are often spared the intestinal damage common in Celiac patients, but suffer from abdominal pain, bloating, diarrhea, constipation, and many “extra-intestinal” symptoms such as “foggy mind”, depression, ADHD-like behavior, headaches, bone or joint pain, and chronic fatigue when they have gluten in their diet.  There are many antigenic triggers (epitopes) in the gluten protein complex that have cytotoxic, immune-modulatory, and gut permeating properties.

Wheat Allergy
Wheat allergy is caused by an individual’s IgE antibody response to many classes of wheat proteins including; serine protease inhibitors, gliadins, glutelins, prolamines and gluten. Symptoms of wheat allergy reaction can range from mild, such as hives, to severe, such as anaphylaxis.

ZRT Lab

1) Adrenal Stress Profile

Saliva testing of DHEA and diurnal cortisol levels measured at four points during the day is the accepted industry standard for assessing full adrenal function. Test results tell us the extent to which stress hormones are out of balance and serve as a guide to effective stress management.
Cortisol is the primary hormone secreted by the adrenals in reaction to stress. Along with DHEA (another essential adrenal hormone) it is involved in the regulation of glucose, insulin, fatty acids, inflammation, bone and muscle building, mood and memory, energy, stamina, and immunities against disease.

Adrenals in balance produce adequate amounts of DHEA and cortisol to power us throughout the day, taking stress in stride. Adrenals out of balance are overworked and are unable to produce enough essential hormones to keep us running on all cylinders. When that happens we start to lose steam, sleep fitfully, get sick, and pack on pounds through the middle.
Cortisol’s main impact on the immune system is double-edged. Acute stress pumps up body defenses, sending white blood cells to their battle stations. Chronic stress weakens immune reserves leaving us vulnerable to illness, inflammation, and any virus that comes along.

Long term Stress Effects
When stress is prolonged, adrenal hormones seesaw triggering blood sugar and insulin imbalances, food cravings, weight gain and sleep disturbances. Adrenals under pressure create imbalances of other hormones, stealing progesterone away from its reproductive duties to make extra cortisol, or by inhibiting thyroid function and metabolism. According to Dr. James Wilson, author of the go-to book, Adrenal Fatigue, “about 80% of adrenal fatigue sufferers also have symptoms of low thyroid.”
If stress levels stay high the adrenals remain in “survival mode” to keep us going, by increasing alertness (i.e., sleeplessness), appetite (i.e., overeating) and fat reserves (i.e., stored as belly fat), while immunities steadily weaken.

2) Salivary Male/Female Hormone Profile

Estrogens, progesterone, testosterone, DHEA-S and cortisol are routinely measured in saliva at ZRT. Steroid hormones in the bloodstream are mostly (95-99%) bound to carrier proteins, and in this form are unavailable to target tissues. Only the unbound fraction freely diffuses into tissues, including the salivary gland. Hormone levels in saliva therefore represent the quantity of the hormone that is currently available to target tissues and actively exerting specific effects on the body.

Female/Male Saliva Profile III measures the Estrogen, Progesterone, Testosterone, DHEA sulphate and Cortisol)
(E2, Pg, T, DS, & C x 4)

Genomic Testing

1) CardioGenomic plus profile:

The CardioGenomic Plus Profile uses genomic testing to identify the risk of genetic susceptibility to cardiac related diseases and conditions. Specifically, the CardioGenomic Plus profile evaluates genetic variations, called single nucleotide polymorphisms (SNPs) in genes that modulate blood pressure regulation, lipid balance, nutrient metabolism, inflammation, and oxidative stress.
This test uncovers potential genetic susceptibility to:
• Hypercholestronemia
• Atherosclerosis
• Obesity
• Hypertension
• Myocardial infarction
• Thrombosis
• Endothelial dysfunction
• Stroke

Specialized genomic testing from Genova Diagnostics can provide a glimpse into ones potential health future.

Genetic testing enables one to minimize the risk by:
• Identifying hidden gene mutations that may promote chronic disease
• Preventing disease through early intervention
• Modifying gene expression through more precise, targeted, individualized interventions
• Identifying key areas for follow-up testing
• Monitoring therapeutic effectiveness of intervention strategies with laboratory testing
Whether or not you choose to see genes, they are always there and will continue to play an important role in one´s health. With genomic testing, by choosing to look at them, you have the opportunity to influence the ultimate outcome and more actively promote a healthy life.

The CardioGenomic Profile evaluations include:

•Cholesterol Regulation and Atherosclerosis
• ApoE (apolipoprotein E)
• CETP (cholesterylester transfer protein)
• SELE (E Selectin)

These genes affect the breaks down of fats and the processing of cholestrol. They also affect lipid balance, plaque formation, and blood vessel integrity and function.

Methylation
• MTHFR (methylenetetrahydrofolate reductase)
Polymorphisms of this enzyme can disrupt the metabolism of homocysteine, resulting in its accumulation as well as impaired methylation. The presence of these SNPs can increase the risk of cardiovascular disease, blood vessel damage, thrombosis (blood clots), stroke, and degenerative aging.

Hypertension
• GNB3 (guanine nucleotide-binding protein)
• AGTR1 (angiotensin II receptor-1)
Polymorphisms of these genes are associated with blood vessel constriction, sodium and water retention, obesity, and increased susceptibility to hypertension.

Coagulation
• Factor 2 (prothrombin)
• Factor 5 (Leiden)
• PAI-1 (Plasminogen activator inhibitor-1)
• GP3a (Glycoprotein 3)
These genetic variants can over-activate blood clotting processes, increasing the risk of sudden cardiac events such as thrombosis, heart attacks, and strokes.
Test Type: Stool Analysis

2) Estrogenomic Profile

The EstroGenomic® Profile uses genomic testing to identify susceptibility to diseases and conditions such as breast cancer, osteoporosis, and heart disease. More specifically, the EstroGenomic® Profile evaluates genetic variations, called single nucleotide polymorphisms (SNPs) in genes that modulate estrogen metabolism, coagulation, cardiovascular function, bone health, and inflammation.

The EstroGenomic® test uncovers potential genetic susceptibility to diseases and conditions such as:
• Breast cancer
• Osteoporosis
• Thrombosis
• Strokes
• Atherosclerosis
• Heart Disease

Specialised genomic testing from Genova Diagnostics can provide a glimpse into one’s potential health future.

Genetic testing enables one to minimize the risk by:
• Identifying hidden gene mutations that may promote chronic disease
• Preventing disease through early intervention
• Modifying gene expression through more precise, targeted, individual interventions
• Identifying key areas for follow-up testing
• Monitoring therapeutic effectiveness of intervention strategies with laboratory testing

Whether or not you choose to see genes, they are always there and will continue to play an important role in one´s health.  With genomic testing, by choosing to look at them, you have the opportunity to influence the ultimate outcome and more actively promote a healthy life. The EstroGenomic® Profile evaluations include:

Estrogen Metabolism

• CYP1A1
• GST (M1 and P1)
• CYP1B1
• COMT (catechol-O-methyl transferase)
Estrogen metabolism SNPs focus on the Phase 1 enzymes involved in the formation of anti- or procarcinogenic metabolites such as 2-hydroxyestrone and 4-hydroxyestrone, respectively, as well as the Phase 2 enzymes responsible for the subsequent metabolism of these compounds. Levels of the various estrogen metabolites modulate risk of both breast cancer and osteoporosis.

Hypercoagulation

• GP3a (Glycoprotein 3)
• Factor 2 (Prothrombin)
• PAI-1 (Plasminogen activator inhibitor-1)
• Factor 5 (Leiden)

These genetic variants focus on estrogens interaction with some of the key constituents of the clot formation and fibrinolysis process, such as clotting factors and inhibitors of fibrinolysis. The SNPs can over-activate blood clotting processes, increasing the risk of sudden cardiac events such as thrombosis, heart attacks, and strokes, and especially among women who take supplemental estrogens and oral contraceptives.

Cardiovascular

• Apo E (apolipoprotein E)
• TNF-alpha
• MTHFR
• IL-6

These genes affect how the body processes cholesterol, responds to inflammation, and metabolises B vitamins. The presence of these SNPs can increase risk of cardiovascular disease, hyperlipidemia, and blood vessel damage.

Osteoporosis

• VDR
• TNF-alpha
• IL-6

Osteoporosis SNPs relate to estrogens influence on inflammation, bone resorption, vitamin D function and bone collagen formation.
Test Type: Saliva Test

To know more about these tests, email or call us.

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